FastQCFastQC Report
Sat 23 Sep 2023
SRR13269805.YAP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR13269805.YAP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33641838
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGAGCTAATCTCGT16542904.917359152612292Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAG15703264.667777069730851Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCGAGATCGGAAGAGCGGTTCAGC2140480.636255367498054Illumina Paired End Adapter 2 (96% over 28bp)
CGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCG881650.26206950999526246Illumina Paired End PCR Primer 2 (100% over 31bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGAGCTAATATCGT496950.14771785061208606Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGGAGCTAAGCTCGT394960.1174014332986206Illumina Paired End Sequencing Primer 2 (100% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCA342480.10180180999623148Illumina Paired End Adapter 2 (100% over 32bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)